Different application volumes of ethyl-cyanoacrylate tissue adhesive can change its antibacterial effects against ocular pathogens in vitro

Curr Eye Res. 2008 Oct;33(10):813-8. doi: 10.1080/02713680802437692.


Purpose: To analyze the antibacterial effects in vitro of ethyl-cyanoacrylate (EC) tissue adhesive in different application volumes.

Methods: Volumes of 4, 6, 8, 10, 12, 14, 25, and 35 mu l of EC were applied onto the surface of monolayer cultures of Staphylococcus aureus (ATCC25924), Streptococcus pneumoniae (ATCC49619), Escherichia coli (ATCC25922), Pseudomonas aeruginosa (ATCC27853), and Klebsiella pneumoniae (ATCC13883). The diameter of each EC drop was measured, and the area of the circle of EC (formed after its application onto the monolayer culture) was calculated. The area of the antibacterial inhibitory halo surrounding the drop of EC on the monolayer culture was calculated. The ratio between the area of the EC and of the corresponding inhibitory halo was obtained for each EC volume and for each of the 5 types of bacteria studied.

Results: EC volume-dependent inhibitory halos were seen in Staphylococcus aureus, Streptococcus pneumoniae, and Escherichia coli, but not in Pseudomonas aeruginosa or Klebsiella pneumoniae.

Conclusion: The in vitro antibacterial effect of EC against Staphylococcus aureus, Streptococcus pneumoniae, and Escherichia coli varies in a dose-dependent fashion with its volume. No effect was observed against Pseudomonas aeruginosa and Klebsiella pneumoniae.

Publication types

  • Comparative Study

MeSH terms

  • Anti-Bacterial Agents / administration & dosage*
  • Bacteria / drug effects*
  • Cyanoacrylates / administration & dosage*
  • Escherichia coli / drug effects
  • Klebsiella pneumoniae / drug effects
  • Microbial Sensitivity Tests
  • Pseudomonas aeruginosa / drug effects
  • Staphylococcus aureus / drug effects
  • Streptococcus pneumoniae / drug effects
  • Tissue Adhesives / administration & dosage*


  • Anti-Bacterial Agents
  • Cyanoacrylates
  • Tissue Adhesives
  • ethyl 2-cyanoacrylate