A novel vector system for gene transfer into the cornea using a partially dried plasmid expressing 18 basic fibroblast growth factor-synthetic amphiphile INTeraction-18 (SAINT-18) complex

Curr Eye Res. 2008 Oct;33(10):839-48. doi: 10.1080/02713680802382963.


Purpose: We describe a novel vector system of nonviral gene transfer into the cornea using a partially dried form of a plasmid expressing 18-kDa basic fibroblast growth factor (p-bFGF)-synthetic amphiphile INTeraction-18 (SAINT-18) complex.

Methods: Corneal neovascularization (NV) was evaluated in 48 eyes of Sprague-Dawley rats after implantation of SAINT-18 containing 2 micro g of plasmid-expressing green fluorescent protein (p-GFP; control group), 0.2 micro g, 2 micro g, or 20 micro g of p-bFGF from day 0 to day 60. bFGF protein expression was analyzed by Western blotting and immunohistochemistry.

Results: The p-bFGF-SAINT-18 complex induced dose-dependent corneal neovascularization, which reached a maximum on days 15-21 in the 20-micro g p-bFGF group, days 12-18 in the 2-micro g p-bFGF group, and on days 9-15 in the 0.2-micro g p-bFGF group, and then regressed progressively. No NV was observed in the p-GFP group.

Conclusions: This noninflammatory corneal transfection model using partially dried p-bFGF-SAINT-18 complex allows precise localization of tranfection reagents for producing corneal neovascularization.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Corneal Neovascularization / genetics*
  • Corneal Neovascularization / pathology
  • Corneal Stroma / metabolism*
  • Fibroblast Growth Factor 2 / genetics*
  • Gene Expression / physiology
  • Gene Transfer Techniques*
  • Genetic Vectors*
  • Immunohistochemistry
  • Male
  • Plasmids / genetics*
  • Pyridinium Compounds / chemistry*
  • Rats
  • Rats, Sprague-Dawley
  • Surface-Active Agents


  • 1-methyl-4-(9-dioleyl)methylpyridinium
  • Pyridinium Compounds
  • Surface-Active Agents
  • Fibroblast Growth Factor 2