A guide to the perplexed on the specificity of antibodies

doi:10.1369/jhc.2008.952770

Review

. 2009 Jan;57(1):1-5.

doi: 10.1369/jhc.2008.952770. Epub 2008 Oct 14.

A guide to the perplexed on the specificity of antibodies

Clifford B Saper¹

Affiliations

PMID: 18854594
PMCID: PMC2605712
DOI: 10.1369/jhc.2008.952770

Review

A guide to the perplexed on the specificity of antibodies

Clifford B Saper. J Histochem Cytochem. 2009 Jan.

. 2009 Jan;57(1):1-5.

doi: 10.1369/jhc.2008.952770. Epub 2008 Oct 14.

Author

Clifford B Saper¹

Affiliation

¹ Department of Neurology, Beth Israel Deaconess Medical Center, 330 Brookline Avenue, Boston, MA 02215, USA. csaper@bidmc.harvard.edu

PMID: 18854594
PMCID: PMC2605712
DOI: 10.1369/jhc.2008.952770

Abstract

Many investigators are unaware of the potential problems with specificity of antibodies and the need to document antibody characterization meticulously for each antibody that is used. In this review, I consider the principles of antibody action and how they define a set of rules for what information should be obtained by the investigator before using an antibody in a serious scientific investigation.

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References

1. Coons AH (1958) Fluorescent antibody methods. Gen Cytochem Methods 1:399–422 - PubMed
1. Fox CH, Johnson FB, Whiting J, Roller PP (1985) Formaldehyde fixation. J Histochem Cytochem 33:845–853 - PubMed
1. Guan N, Yu LX, Wu GH, Xing Y, Ding J (2008) Antigen retrieval with protease digestion applied in immunohistochemical diagnosis of Alport syndrome. Nephrol Dial Transplant 23:3509–3513 - PMC - PubMed
1. Long DJ, Buggs C (2008) Microwave oven-based technique for immunofluorescent staining of paraffin-embedded tissues. J Mol Histol 39:1–4 - PMC - PubMed
1. Lorincz A, Nusser Z (2008) Specificity of immunoreactions: the importance of testing specificity in each method. J Neurosci 28:9083–9086 - PMC - PubMed

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[1] Coons AH (1958) Fluorescent antibody methods. Gen Cytochem Methods 1:399–422 - PubMed

[2] Coons AH (1958) Fluorescent antibody methods. Gen Cytochem Methods 1:399–422 - PubMed

[3] Fox CH, Johnson FB, Whiting J, Roller PP (1985) Formaldehyde fixation. J Histochem Cytochem 33:845–853 - PubMed

[4] Fox CH, Johnson FB, Whiting J, Roller PP (1985) Formaldehyde fixation. J Histochem Cytochem 33:845–853 - PubMed

[5] Guan N, Yu LX, Wu GH, Xing Y, Ding J (2008) Antigen retrieval with protease digestion applied in immunohistochemical diagnosis of Alport syndrome. Nephrol Dial Transplant 23:3509–3513 - PMC - PubMed

[6] Guan N, Yu LX, Wu GH, Xing Y, Ding J (2008) Antigen retrieval with protease digestion applied in immunohistochemical diagnosis of Alport syndrome. Nephrol Dial Transplant 23:3509–3513 - PMC - PubMed

[7] Long DJ, Buggs C (2008) Microwave oven-based technique for immunofluorescent staining of paraffin-embedded tissues. J Mol Histol 39:1–4 - PMC - PubMed

[8] Long DJ, Buggs C (2008) Microwave oven-based technique for immunofluorescent staining of paraffin-embedded tissues. J Mol Histol 39:1–4 - PMC - PubMed

[9] Lorincz A, Nusser Z (2008) Specificity of immunoreactions: the importance of testing specificity in each method. J Neurosci 28:9083–9086 - PMC - PubMed

[10] Lorincz A, Nusser Z (2008) Specificity of immunoreactions: the importance of testing specificity in each method. J Neurosci 28:9083–9086 - PMC - PubMed

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A guide to the perplexed on the specificity of antibodies

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A guide to the perplexed on the specificity of antibodies

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