Detection of the Lyme disease bacterium, Borrelia burgdorferi, by using the polymerase chain reaction and a nonradioisotopic gene probe

J Clin Microbiol. 1991 Jul;29(7):1523-6. doi: 10.1128/jcm.29.7.1523-1526.1991.

Abstract

A 419-bp region of the flagellin gene sequence of Borrelia burgdorferi was used as a target for the polymerase chain reaction. With a nonradioactively labeled gene-specific probe, sensitivity to as few as 1 to 10 spirochetes was observed. The targeted gene fragment was conserved in the American and European strains of B. burgdorferi tested and among several other pathogenic borreliae.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Borrelia burgdorferi Group / genetics
  • Borrelia burgdorferi Group / isolation & purification*
  • DNA Probes
  • DNA, Bacterial / genetics
  • Evaluation Studies as Topic
  • Flagellin / genetics
  • Genes, Bacterial
  • Humans
  • Lyme Disease / diagnosis
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity

Substances

  • DNA Probes
  • DNA, Bacterial
  • Flagellin