Abstract
A 419-bp region of the flagellin gene sequence of Borrelia burgdorferi was used as a target for the polymerase chain reaction. With a nonradioactively labeled gene-specific probe, sensitivity to as few as 1 to 10 spirochetes was observed. The targeted gene fragment was conserved in the American and European strains of B. burgdorferi tested and among several other pathogenic borreliae.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Borrelia burgdorferi Group / genetics
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Borrelia burgdorferi Group / isolation & purification*
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DNA Probes
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DNA, Bacterial / genetics
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Evaluation Studies as Topic
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Flagellin / genetics
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Genes, Bacterial
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Humans
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Lyme Disease / diagnosis
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Molecular Sequence Data
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Polymerase Chain Reaction / methods*
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Sensitivity and Specificity
Substances
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DNA Probes
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DNA, Bacterial
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Flagellin