Simple disk-based method for detection of Klebsiella pneumoniae carbapenemase-type beta-lactamase by use of a boronic acid compound

J Clin Microbiol. 2008 Dec;46(12):4083-6. doi: 10.1128/JCM.01408-08. Epub 2008 Oct 15.


A disk potentiation method using carbapenems as substrates and 3-aminophenyl boronic acid as an inhibitor was evaluated for the detection of Klebsiella pneumoniae carbapenemase (KPC)-type beta-lactamases. When combined with nonsusceptibility to ertapenem, the method was easy to perform and reliably differentiated isolates producing KPC-type beta-lactamases from those producing other types of beta-lactamases.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Boronic Acids / pharmacology*
  • Carbapenems / metabolism*
  • Enzyme Inhibitors / pharmacology*
  • Humans
  • Klebsiella pneumoniae / drug effects
  • Klebsiella pneumoniae / enzymology*
  • Microbial Sensitivity Tests / methods*
  • beta-Lactam Resistance
  • beta-Lactamases / biosynthesis*


  • Boronic Acids
  • Carbapenems
  • Enzyme Inhibitors
  • beta-Lactamases