Combined multilocus short-sequence-repeat and mycobacterial interspersed repetitive unit-variable-number tandem-repeat typing of Mycobacterium avium subsp. paratuberculosis isolates

J Clin Microbiol. 2008 Dec;46(12):4091-4. doi: 10.1128/JCM.01349-08. Epub 2008 Oct 15.

Abstract

Short-sequence-repeat (SSR) sequencing was applied to 127 Mycobacterium avium subsp. paratuberculosis isolates typed by mycobacterial interspersed repetitive unit-variable-number tandem repeats (MIRU-VNTR) and IS900 restriction fragment length polymorphism (RFLP). Combined MIRU-VNTR and SSR typing followed by secondary IS900 RFLP typing is an improved approach to high-resolution genotyping of this pathogen.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques / methods*
  • DNA Fingerprinting / methods*
  • DNA, Bacterial / genetics*
  • Mycobacterium avium subsp. paratuberculosis / classification*
  • Mycobacterium avium subsp. paratuberculosis / genetics*
  • Mycobacterium avium subsp. paratuberculosis / isolation & purification
  • Paratuberculosis / microbiology*
  • Repetitive Sequences, Nucleic Acid / genetics*

Substances

  • DNA, Bacterial