Expression patterns of the lysophospholipid receptor genes during mouse early development

Dev Dyn. 2008 Nov;237(11):3280-94. doi: 10.1002/dvdy.21736.


Lysophospholipids (LPs) such as lysophosphatidic acid (LPA) and sphingosine-1-phosphate (S1P) are known to mediate various biological responses, including cell proliferation, migration, and differentiation. To better understand the role of these lipids in mammalian early development, we applied whole-mount in situ hybridization techniques to E8.5 to E12.5 mouse embryos. We determined the expression patterns of the following LP receptor genes, which belong to the G protein-coupled receptor (GPCR) family: EDG1 to EDG8 (S1P1 to S1P5 and LPA1 to LPA3), LPA4 (GPR23/P2Y9), and LPA5 (GPR92). We found that the S1P/LPA receptor genes exhibit overlapping expression patterns in a variety of organ primordia, including the developing brain and cardiovascular system, presomitic mesoderm and somites, branchial arches, and limb buds. These results suggest that multiple receptor systems for LPA/S1P lysophospholipids may be functioning during organogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Gene Expression Regulation, Developmental / physiology*
  • Lysophospholipids / metabolism*
  • Mice
  • Organ Specificity / physiology
  • Organogenesis / physiology*
  • Receptors, Lysophosphatidic Acid / biosynthesis*
  • Sphingosine / analogs & derivatives*
  • Sphingosine / metabolism


  • Lysophospholipids
  • Receptors, Lysophosphatidic Acid
  • sphingosine 1-phosphate
  • Sphingosine
  • lysophosphatidic acid