Studying isoform-specific inositol 1,4,5-trisphosphate receptor function and regulation

Methods. 2008 Nov;46(3):177-82. doi: 10.1016/j.ymeth.2008.09.014. Epub 2008 Oct 16.


Inositol 1,4,5-trisphosphate receptors (InsP3R) are a family of ubiquitously expressed intracellular Ca2+ channels. Isoform-specific properties of the three family members may play a prominent role in defining the rich diversity of the spatial and temporal characteristics of intracellular Ca2+ signals. Studying the properties of the particular family members is complicated because individual receptor isoforms are typically never expressed in isolation. In this article, we discuss strategies for studying Ca2+ release through individual InsP3R family members with particular reference to methods applicable following expression of recombinant InsP3R and mutant constructs in the DT40-3KO cell line, an unambiguously null InsP3R expression system.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Calcium / metabolism*
  • Calcium Signaling / physiology*
  • Cell Line, Tumor
  • Chickens
  • Fura-2 / analogs & derivatives
  • Fura-2 / metabolism
  • Inositol 1,4,5-Trisphosphate Receptors / genetics
  • Inositol 1,4,5-Trisphosphate Receptors / physiology*
  • Permeability
  • Photolysis
  • Protein Isoforms / metabolism
  • Rats


  • Inositol 1,4,5-Trisphosphate Receptors
  • Protein Isoforms
  • 2-(2-(5-carboxy)oxazole)-5-hydroxy-6-aminobenzofuran-N,N,O-triacetic acid
  • Calcium
  • Fura-2