Characterization of a Bacillus Subtilis 64-kDa DNA Polymerase X Potentially Involved in DNA Repair

J Mol Biol. 2008 Dec 31;384(5):1019-28. doi: 10.1016/j.jmb.2008.09.081. Epub 2008 Oct 10.

Abstract

Bacillus subtilis gene yshC encodes a 64-kDa family X DNA polymerase (PolXBs), which contains all the critical residues involved in DNA and nucleotide binding as well as those responsible for catalysis of DNA polymerization, conserved in most family X members. Biochemical analyses of the purified enzyme indicate that PolXBs is a monomeric and strictly template-directed DNA polymerase, preferentially acting on DNA structures containing gaps from one to a few nucleotides and bearing a phosphate group at the 5' end of the downstream DNA. The fact that PolXBs is able to conduct filling of a single-nucleotide gap, allowing further sealing of the resulting nick by a DNA ligase, points to a putative role in base excision repair during the B. subtilis life cycle.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Archaea / enzymology
  • Bacillus subtilis / enzymology*
  • DNA Polymerase beta / chemistry
  • DNA Repair*
  • DNA, Bacterial / metabolism
  • DNA-Directed DNA Polymerase / chemistry
  • DNA-Directed DNA Polymerase / metabolism*
  • Molecular Sequence Data
  • Molecular Weight
  • Nucleotides
  • Sequence Alignment
  • Substrate Specificity

Substances

  • DNA, Bacterial
  • Nucleotides
  • DNA Polymerase beta
  • DNA polymerase X
  • DNA-Directed DNA Polymerase