A high-throughput assay for siRNA-based circadian screens in human U2OS cells

PLoS One. 2008;3(10):e3457. doi: 10.1371/journal.pone.0003457. Epub 2008 Oct 20.


The advent of siRNA-based screens has revolutionized the efficiency by which functional components of biological processes are identified. A notable exception has been the field of mammalian circadian rhythms. Here, we outline a medium- to high-throughput siRNA-based approach that, in combination with real-time bioluminescence measurement of a circadian reporter gene, can be utilized to elucidate the effects of gene knockdown across several days in human cells.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Circadian Rhythm / genetics*
  • Drug Evaluation, Preclinical / methods*
  • Gene Silencing
  • Genes, Reporter
  • Humans
  • Luminescent Measurements
  • Methods
  • RNA, Small Interfering / pharmacology*


  • RNA, Small Interfering