Efficient temporally-controlled targeted mutagenesis in smooth muscle cells of the adult mouse

Genesis. 2009 Jan;47(1):14-8. doi: 10.1002/dvg.20448.


To generate temporally-controlled targeted somatic mutations selectively and efficiently in smooth muscles, we have established a transgenic SMA-Cre-ER(T2) mouse line in which the expression of the Tamoxifen-dependent Cre-ER(T2) recombinase is under the control of a large genomic DNA segment of the mouse smooth muscle alpha actin (SMA) gene, contained in a Bacterial artificial chromosome (Bac). In this transgenic mouse line, Cre-ER(T2)-mediated recombination of LoxP-flanked target DNA is strictly Tamoxifen-dependent, and efficient in both vascular and visceral smooth muscle cells. Moreover, with the exception of few cardiomyocytes, LoxP-flanked DNA excision is restricted to smooth muscle cells. Thus, SMA-Cre-ER(T2) mice should be of great value to analyze gene function in smooth muscles, and to establish new animal models of human smooth muscle disorders.

MeSH terms

  • Aging / physiology*
  • Animals
  • Genetic Engineering / methods*
  • Mice
  • Mice, Transgenic
  • Mutagenesis / genetics*
  • Myocytes, Smooth Muscle / metabolism*
  • Time Factors