Comparison of pertussis toxin (PT)-neutralizing activities and mouse-protective activities of anti-PT mouse monoclonal antibodies

H Sato; Y Sato; I Ohishi

doi:10.1128/iai.59.10.3832-3835.1991

Comparison of pertussis toxin (PT)-neutralizing activities and mouse-protective activities of anti-PT mouse monoclonal antibodies

Infect Immun. 1991 Oct;59(10):3832-5. doi: 10.1128/iai.59.10.3832-3835.1991.

Authors

H Sato¹, Y Sato, I Ohishi

Affiliation

¹ Department of Applied Immunology, National Institute of Health, Tokyo, Japan.

Abstract

An assay of neutralizing activity against CHO cell-clustering activity, one of the most common assays for anti-pertussis toxin antibodies, did not correlate well with an assay of the protective activities of monoclonal antibodies. A better correlation between neutralization of leukocytosis-promoting or islet-activating activity and mouse protection against aerosol challenge was seen.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Adenosine Diphosphate Ribose / metabolism
Animals
Antibodies, Monoclonal / immunology*
Cricetinae
Leukocytosis / chemically induced
Mice
Pertussis Toxin*
Virulence Factors, Bordetella / immunology*
Virulence Factors, Bordetella / toxicity
Whooping Cough / prevention & control*

Substances

Antibodies, Monoclonal
Virulence Factors, Bordetella
Adenosine Diphosphate Ribose
Pertussis Toxin