Comparison of pertussis toxin (PT)-neutralizing activities and mouse-protective activities of anti-PT mouse monoclonal antibodies

Infect Immun. 1991 Oct;59(10):3832-5. doi: 10.1128/iai.59.10.3832-3835.1991.


An assay of neutralizing activity against CHO cell-clustering activity, one of the most common assays for anti-pertussis toxin antibodies, did not correlate well with an assay of the protective activities of monoclonal antibodies. A better correlation between neutralization of leukocytosis-promoting or islet-activating activity and mouse protection against aerosol challenge was seen.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Diphosphate Ribose / metabolism
  • Animals
  • Antibodies, Monoclonal / immunology*
  • Cricetinae
  • Leukocytosis / chemically induced
  • Mice
  • Pertussis Toxin*
  • Virulence Factors, Bordetella / immunology*
  • Virulence Factors, Bordetella / toxicity
  • Whooping Cough / prevention & control*


  • Antibodies, Monoclonal
  • Virulence Factors, Bordetella
  • Adenosine Diphosphate Ribose
  • Pertussis Toxin