Effect of neutralizing sera on factor x-mediated adenovirus serotype 5 gene transfer

J Virol. 2009 Jan;83(1):479-83. doi: 10.1128/JVI.01878-08. Epub 2008 Oct 22.


The deployment of adenovirus serotype 5 (Ad5)-based vectors is hampered by preexisting immunity. When such vectors are delivered intravenously, hepatocyte transduction is mediated by the hexon-coagulation factor X (FX) interaction. Here, we demonstrate that human sera efficiently block FX-mediated cellular binding and transduction of Ad5-based vectors in vitro. Neutralizing activity correlated well with the ability to inhibit Ad5-mediated liver transduction, suggesting that prescreening patient sera in this manner accurately predicts the efficacy of Ad5-based gene therapies. Neutralization in vitro can be partially bypassed by pseudotyping with Ad45 fiber protein, indicating that a proportion of neutralizing antibodies are directed against the Ad5 fiber.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoviridae / immunology*
  • Cell Line, Tumor
  • Factor X / metabolism*
  • Genetic Therapy / methods
  • Genetic Vectors / immunology*
  • Hepatocytes / virology*
  • Humans
  • Immune Sera / metabolism*
  • Neutralization Tests
  • Transduction, Genetic
  • Virus Attachment


  • Immune Sera
  • Factor X