Cytotoxic and genotoxic effects of [Ru(phi)3]2+ evaluated by Ames/Salmonella and MTT methods

Eur J Med Chem. 2009 Jun;44(6):2601-5. doi: 10.1016/j.ejmech.2008.09.017. Epub 2008 Sep 24.

Abstract

In this work, we synthesized and evaluated the cytotoxic effect of [Ru(phi)(3)](2+), on rat C6 glioma cell line. Cell viability was determined by assay with 3-(4-5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT). The mutagenicity of [Ru(phi)(3)](2+) was studied in vitro by using two strains of Salmonella typhimurium with frameshift mutation (TA98) and base-pair substitution mutation (TA100) were used in plate incorporation assay in the absence of metabolic activation. According to the results, the Ru compound is not toxic but mutagenic, and it shows cytotoxic effect towards C6 rat glioma cells in 100 microM.

Publication types

  • Evaluation Study

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Cell Survival / drug effects
  • DNA Repair / drug effects
  • Dose-Response Relationship, Drug
  • Drug Screening Assays, Antitumor
  • Frameshift Mutation
  • Glioma / drug therapy*
  • Glioma / pathology
  • Molecular Structure
  • Mutagenicity Tests
  • Organometallic Compounds / chemical synthesis
  • Organometallic Compounds / chemistry
  • Organometallic Compounds / pharmacology*
  • Rats
  • Salmonella typhimurium / drug effects*
  • Salmonella typhimurium / genetics*
  • Tetrazolium Salts / chemistry*
  • Thiazoles / chemistry*

Substances

  • Organometallic Compounds
  • Tetrazolium Salts
  • Thiazoles
  • tris(9,10-phenanthrenequinone diimine)ruthenium(II)
  • thiazolyl blue