Percent positive rate of Lyme real-time polymerase chain reaction in blood, cerebrospinal fluid, synovial fluid, and tissue

Diagn Microbiol Infect Dis. 2008 Dec;62(4):464-6. doi: 10.1016/j.diagmicrobio.2008.08.016. Epub 2008 Oct 22.


We performed a retrospective review of 23,777 specimens tested by our Lyme real-time polymerase chain reaction assay to determine the percent positive rates by specimen source. The percent positive rates were highest in synovial fluid (6.4%) and tissue (6.5%), and lowest in blood (0.1%) and cerebrospinal fluid (0.09%).

MeSH terms

  • Bacterial Proteins / analysis
  • Borrelia burgdorferi / isolation & purification*
  • Cerebrospinal Fluid / microbiology
  • Humans
  • Lyme Disease*
  • Polymerase Chain Reaction / methods*
  • Reproducibility of Results
  • Retrospective Studies
  • Synovial Fluid / microbiology


  • Bacterial Proteins