Effects of alcohol-free beer on lipid profile and parameters of oxidative stress and inflammation in elderly women

Nutrition. 2009 Feb;25(2):182-7. doi: 10.1016/j.nut.2008.08.005. Epub 2008 Oct 23.


Objective: We assessed the influence of alcohol-free beer on factors implicated in atherosclerosis, such as lipid profile, oxidative stress parameters, and proinflammatory cytokines, in postmenopausal women, a population particularly at risk for atherosclerotic disease.

Methods: The study was carried out in 29 nuns, 58 to 73 y old, who live in a convent with a disciplined, regular, and homogeneous lifestyle. The nuns maintained their habits and diet routine, but their meals were supplemented with 500 mL/d of alcohol-free beer (0.0%) divided into two doses over a 45-d period. Lipid profile, inflammatory markers such as C-reactive protein, interleukins 1 and 6, and tumor necrosis factor-alpha, and parameters of oxidative metabolism were determined before and after the study period.

Results: There were no differences in the levels of C-reactive protein and proinflammatory cytokines after diet supplementation. The antibody titers to oxidized low-density lipoprotein were significant lower (P < 0.05), and thiobarbituric acid-reactive substances (-18%, P < 0.001) and plasma carbonyl group content (-21%, P < 0.001) were decreased when compared with initial values. Increases in alpha-tocopherol levels (+9%, P < 0.05) and erythrocytic glutathione levels (+29%, P < 0.001) were also noted. With respect to lipid profile, only subjects with cholesterol levels higher than 240 mg/dL showed lower levels after supplementation.

Conclusion: Consumption of non-alcoholic beer produces a decrease in oxidative stress that can have a beneficial impact on cardiovascular risk; however, the circulating concentrations of inflammatory mediators involved in its pathophysiology remained unchanged.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Beer*
  • Beverages
  • Biomarkers / blood
  • C-Reactive Protein / metabolism
  • Cardiovascular Diseases / blood
  • Cardiovascular Diseases / epidemiology
  • Cardiovascular Diseases / prevention & control
  • Ethanol / administration & dosage
  • Female
  • Humans
  • Inflammation / blood*
  • Inflammation Mediators / blood*
  • Interleukins / blood
  • Lipid Peroxidation
  • Lipids / blood*
  • Middle Aged
  • Oxidation-Reduction
  • Oxidative Stress / drug effects*
  • Postmenopause / blood
  • Thiobarbituric Acid Reactive Substances / metabolism
  • Tumor Necrosis Factor-alpha / blood


  • Biomarkers
  • Inflammation Mediators
  • Interleukins
  • Lipids
  • Thiobarbituric Acid Reactive Substances
  • Tumor Necrosis Factor-alpha
  • Ethanol
  • C-Reactive Protein