Prelamin A is involved in early steps of muscle differentiation

Exp Cell Res. 2008 Dec 10;314(20):3628-37. doi: 10.1016/j.yexcr.2008.09.026. Epub 2008 Oct 11.


Lamin A is a nuclear lamina constituent implicated in a number of human disorders including Emery-Dreifuss muscular dystrophy. Since increasing evidence suggests a role of the lamin A precursor in nuclear functions, we investigated the processing of prelamin A during differentiation of C2C12 mouse myoblasts. We show that both protein levels and cellular localization of prelamin A are modulated during myoblast activation. Similar changes of lamin A-binding proteins emerin and LAP2alpha were observed. Furthermore, prelamin A was found in a complex with LAP2alpha in differentiating myoblasts. Prelamin A accumulation in cycling myoblasts by expressing unprocessable mutants affected LAP2alpha and PCNA amount and increased caveolin 3 mRNA and protein levels, while accumulation of prelamin A in differentiated muscle cells following treatment with a farnesyl transferase inhibitor appeared to inhibit caveolin 3 expression. Our data provide evidence for a critical role of the lamin A precursor in the early steps of muscle cell differentiation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Caveolin 3 / genetics
  • Caveolin 3 / metabolism
  • Cell Differentiation / genetics*
  • Cells, Cultured
  • DNA-Binding Proteins / metabolism
  • Gene Expression Regulation, Developmental
  • Humans
  • Lamin Type A
  • Membrane Proteins / metabolism
  • Mice
  • Muscle Development / genetics*
  • Muscle, Skeletal / metabolism
  • Muscle, Skeletal / physiology
  • Myoblasts / metabolism
  • Myoblasts / physiology
  • Nuclear Proteins / metabolism
  • Nuclear Proteins / physiology*
  • Protein Binding
  • Protein Precursors / metabolism
  • Protein Precursors / physiology*
  • Protein Processing, Post-Translational / physiology
  • Time Factors


  • Caveolin 3
  • DNA-Binding Proteins
  • Lamin Type A
  • Membrane Proteins
  • Nuclear Proteins
  • Protein Precursors
  • lamina-associated polypeptide 2
  • prelamin A