Structural basis for exon recognition by a group II intron

Navtej Toor; Kanagalaghatta Rajashankar; Kevin S Keating; Anna Marie Pyle

doi:10.1038/nsmb.1509

Structural basis for exon recognition by a group II intron

Nat Struct Mol Biol. 2008 Nov;15(11):1221-2. doi: 10.1038/nsmb.1509. Epub 2008 Oct 26.

Authors

Navtej Toor¹, Kanagalaghatta Rajashankar, Kevin S Keating, Anna Marie Pyle

Affiliation

¹ Department of Molecular Biophysics and Biochemistry, Yale University, 266 Whitney Avenue, New Haven, Connecticut 06520, USA.

Abstract

Free group II introns are infectious retroelements that can bind and insert themselves into RNA and DNA molecules via reverse splicing. Here we report the 3.4-A crystal structure of a complex between an oligonucleotide target substrate and a group IIC intron, as well as the refined free intron structure. The structure of the complex reveals the conformation of motifs involved in exon recognition by group II introns.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Crystallography, X-Ray
Exons*
Introns*
Models, Molecular
Molecular Sequence Data
Nucleic Acid Conformation*

Associated data

PDB/3EOG
PDB/3EOH

Abstract

Publication types

MeSH terms

Associated data

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