Effect of profound hypothermia on genomics of hippocampus following complete cerebral ischemia in rats

Neurol Res. 2008 Jun;30(5):536-41. doi: 10.1179/174313208X284250.


Objective: To determine differential gene expression of hippocampus in rats following complete cerebral ischemia with treatment of profound hypothermia compared to normothermia.

Methods: Six rats got 5 minutes of complete cerebral ischemia with circulatory arrest and randomly divided into two groups: normothermia ischemia group (37 +/- 0.3 degrees C, n = 3) and profound hypothermia ischemia group (18 +/- 0.5 degrees C, n = 3). Affymetrix U34A rat arrays were applied to detect the difference of gene expression profile in hippocampus between the two groups.

Results: Expression profiles of a total of 75 transcripts in the profound hypothermia ischemia group were statistically different from those of the normothermia ischemia group, and 33 of them were significantly up-regulated and other 42 were significantly down-regulated (p < 0.07).

Conclusions: Compared with normothermia, profound hypothermia had a significant effect on the gene expression profiles following complete cerebral ischemia, which may be involved in the mechanisms of cerebral protection by profound hypothermia.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain Ischemia / pathology*
  • Brain Ischemia / therapy*
  • Databases, Genetic / statistics & numerical data
  • Gene Expression Profiling / methods
  • Gene Expression Regulation / physiology*
  • Genomics / methods*
  • Hippocampus / metabolism*
  • Hypothermia, Induced / methods*
  • Oligonucleotide Array Sequence Analysis / methods
  • Random Allocation
  • Rats