Regulation of the human PDZK1 expression by peroxisome proliferator-activated receptor alpha

FEBS Lett. 2008 Nov 26;582(28):3884-8. doi: 10.1016/j.febslet.2008.10.027. Epub 2008 Oct 26.

Abstract

Although PDZK1 is a well-known adaptor protein, the mechanisms for its role in transcriptional regulation are largely unknown. The peroxisome proliferator-activated receptor alpha (PPARalpha) is a ligand-activated transcription factor that plays an important role in the regulation of lipid homeostasis. Previously, we established a tetracycline-regulated human cell line that can be induced to express PPARalpha and identified candidate target genes, one of which was PDZK1. In this study, we cloned and characterized the promoter region of the human pdzk1 gene and determined the PPAR response element. Finally, we demonstrate that endogenous PPARalpha regulates PDZK1 expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 5' Flanking Region
  • Base Sequence
  • Carrier Proteins / genetics*
  • Cell Line
  • Humans
  • Membrane Proteins
  • Molecular Sequence Data
  • PPAR alpha / metabolism*
  • Promoter Regions, Genetic / drug effects
  • Tetracycline / pharmacology
  • Transcription Initiation Site
  • Transcription, Genetic
  • Transcriptional Activation*

Substances

  • Carrier Proteins
  • Membrane Proteins
  • PDZK1 protein, human
  • PPAR alpha
  • Tetracycline