Glu43 is an essential residue for coordinating the [Fe2S2] cluster of IscR from Acidithiobacillus ferrooxidans

Jia Zeng; Xiaojian Zhang; Yiping Wang; Chenbing Ai; Qing Liu; Guanzhou Qiu

doi:10.1016/j.febslet.2008.09.060

Glu43 is an essential residue for coordinating the [Fe2S2] cluster of IscR from Acidithiobacillus ferrooxidans

FEBS Lett. 2008 Nov 26;582(28):3889-92. doi: 10.1016/j.febslet.2008.09.060. Epub 2008 Oct 26.

Authors

Jia Zeng¹, Xiaojian Zhang, Yiping Wang, Chenbing Ai, Qing Liu, Guanzhou Qiu

Affiliation

¹ Department of Bioengineering, School of Resources Processing and Bioengineering, Central South University, Changsha, Hunan 410083, PR China. zengjcsu@yahoo.com.cn

PMID: 18955052
DOI: 10.1016/j.febslet.2008.09.060

Abstract

The iron-sulfur cluster regulator (IscR) was reported to be a repressor of the iscRSUA operon. In vitro transcription reactions revealed that the IscR had a repression effect on the iscR promoter. The IscR contains a [Fe2S2] cluster per each monomer, and three highly conserved cysteines were identified to ligate the [Fe2S2] cluster. It was proposed that a non-cysteine residue might be the fourth ligand for the [Fe2S2] cluster. In this study, using site-directed mutagenesis, Glu43 was found to be the fourth residue that coordinates the [Fe2S2] cluster of IscR.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acidithiobacillus / metabolism*
Amino Acid Sequence
Bacterial Proteins / genetics
Bacterial Proteins / metabolism*
Conserved Sequence
Electron Spin Resonance Spectroscopy
Glutamic Acid / genetics
Glutamic Acid / metabolism*
Iron-Sulfur Proteins / genetics
Iron-Sulfur Proteins / metabolism*
Ligands
Molecular Sequence Data
Mutation

Substances

Bacterial Proteins
Iron-Sulfur Proteins
Ligands
Glutamic Acid