Selective and sensitive fluorometric determinations of cobalt(II) and hydrogen peroxide with fluorescein-hydrazide

I Mori; K Takasaki; Y Fujita; T Matsuo

doi:10.1016/s0039-9140(98)00118-0

Selective and sensitive fluorometric determinations of cobalt(II) and hydrogen peroxide with fluorescein-hydrazide

Talanta. 1998 Nov;47(3):631-7. doi: 10.1016/s0039-9140(98)00118-0.

Authors

I Mori¹, K Takasaki, Y Fujita, T Matsuo

Affiliation

¹ Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki Osaka 569-1094, Japan.

PMID: 18967365
DOI: 10.1016/s0039-9140(98)00118-0

Abstract

Fluorophotometric determinations of cobalt(II) and hydrogen peroxide were investigated by using the fluorescence reaction between fluorescein-hydrazide (fl-NHNH(2)), and/or hydrogen peroxide, cobalt(II), respectively. The calibration graphs were liner in the range of 0-6.0 ng cobalt(II) and 0-1000 ng hydrogen peroxide per 10 ml at an emission wavelength (E(m)) of 530 nm with an exicitation wavelength (E(x)) of 508 nm, respectively. These proposed methods were selective and simple, and the effect of foreign ions was negligible in comparison with conventional reported methods such as nitroso R,4-(2-pyridylazo)resorcinol(PAR), alizarin, pyridine-2-aldehyde-2-pyridinehydrazone, stilbazo-cobalt(II), etc.