Human serum albumen enhanced resonance light scattering of dyes

Talanta. 2004 Oct 8;64(2):355-60. doi: 10.1016/j.talanta.2004.02.030.


For the first time, the relationship between the structural parameters of dye molecule and the enhanced resonance light scattering (RLS) of dye-human serum albumen (HSA) was investigated using chemometrics technique. The data set of the enhanced RLS intensity of the dye-HSA was obtained under the same experimental conditions for 22 dyes. After calculated with HyperChem software and selected by stepwise regression approach, six structural parameters for the maximum RLS wavelength were used in multivariable linear regression (MLR) (R=0.932), seven structural parameters for the corresponding enhanced RLS intensity were employed by MLR (R=0.934). According to these models, the maximum RLS wavelength and the enhanced RLS intensity are both attributed to the spatial structure and energies of dye.