On the use of dispersed nanoparticles modified with single layer beta-cyclodextrin as chiral selecor to enhance enantioseparation of clenbuterol with capillary electrophoresis

Na Na; Yuping Hu; Jin Ouyang; Willy R G Baeyens; Joris R Delanghe; Youri E C Taes; Mengxia Xie; Huaying Chen; Yiping Yang

doi:10.1016/j.talanta.2005.11.022

On the use of dispersed nanoparticles modified with single layer beta-cyclodextrin as chiral selecor to enhance enantioseparation of clenbuterol with capillary electrophoresis

Talanta. 2006 Jun 15;69(4):866-72. doi: 10.1016/j.talanta.2005.11.022. Epub 2005 Dec 7.

Authors

Na Na¹, Yuping Hu, Jin Ouyang, Willy R G Baeyens, Joris R Delanghe, Youri E C Taes, Mengxia Xie, Huaying Chen, Yiping Yang

Affiliation

¹ Department of Chemistry, Beijing Normal University, 100875 Beijing, PR China.

PMID: 18970649
DOI: 10.1016/j.talanta.2005.11.022

Abstract

A new strategy for chiral separation by capillary electrophoresis employing modified-nanoparticles as chiral selector is described for clenbuterol analysis. Nanoparticles modified with beta-cyclodextrin (beta-CD) form a large surface area platform to serve as a pseudostationary chiral phase, which can be applied for the enhancement of the enantioseparation. The application of four kinds of nanoparticles was investigated (multi-walled nanotubes (MWNTs), polystyrene (PS), TiO(2) and Al(2)O(3)) modified with single layer beta-CD as chiral selector in the enantioseparation of clenbuterol by capillary electrophoresis (CE). Successful clenbuterol enantioseparation could be achieved with the beta-CD-modified MWNTs as chiral selector. X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) confirmed the beta-CD modification of the nanoparticles. The effects of nanoparticles, surfactant, chiral selector (beta-CD) and run buffer were studied in relation to the enantiomeric separation of clenbuterol. This study opens attractive perspectives for the use of modified nanoparticles for chiral separational purposes in CE.