Proliferation-promoting effect of platelet-rich plasma on human adipose-derived stem cells and human dermal fibroblasts

Plast Reconstr Surg. 2008 Nov;122(5):1352-60. doi: 10.1097/PRS.0b013e3181882046.

Abstract

Background: This study evaluated changes in platelet-derived growth factor (PDGF)-AB and transforming growth factor (TGF)-beta1 release from platelets by platelet-rich plasma activation, and the proliferation potential of activated platelet-rich plasma and platelet-poor plasma on human adipose-derived stem cells and human dermal fibroblasts.

Methods: Platelet-rich plasma was prepared using a double-spin method, with the number of platelets counted in each preparation stage. Platelet-rich and platelet-poor plasma were activated with autologous thrombin and calcium chloride, and levels of platelet-released PDGF-AB and TGF-beta1 were determined by enzyme-linked immunosorbent assay. Cells were cultured for 1, 4, or 7 days in serum-free Dulbecco's Modified Eagle Medium supplemented with 5% whole blood plasma, nonactivated platelet-rich plasma, nonactivated platelet-poor plasma, activated platelet-rich plasma, or activated platelet-poor plasma. In parallel, these cells were cultured for 1, 4, or 7 days in serum-free Dulbecco's Modified Eagle Medium supplemented with 1%, 5%, 10%, or 20% activated platelet-rich plasma. The cultured human adipose-derived stem cells and human dermal fibroblasts were assayed for proliferation.

Results: Platelet-rich plasma contained approximately 7.9 times as many platelets as whole blood, and its activation was associated with the release of large amounts of PDGF-AB and TGF-beta1. Adding activated platelet-rich or platelet-poor plasma significantly promoted the proliferation of human adipose-derived stem cells and human dermal fibroblasts. Adding 5% activated platelet-rich plasma to the medium maximally promoted cell proliferation, but activated platelet-rich plasma at 20% did not promote it.

Conclusions: Platelet-rich plasma can enhance the proliferation of human adipose-derived stem cells and human dermal fibroblasts. These results support clinical platelet-rich plasma application for cell-based, soft-tissue engineering and wound healing.

MeSH terms

  • Adipocytes / cytology*
  • Adult Stem Cells / cytology*
  • Blood Platelets / drug effects
  • Blood Platelets / metabolism
  • Calcium Chloride / pharmacology
  • Cell Culture Techniques / methods*
  • Cell Division / physiology
  • Cells, Cultured
  • Dermis / cytology
  • Fibroblasts / cytology*
  • Humans
  • Platelet Activation
  • Platelet Count
  • Platelet-Derived Growth Factor / metabolism
  • Platelet-Rich Plasma*
  • Subcutaneous Fat / cytology
  • Thrombin / pharmacology
  • Transforming Growth Factor beta1 / metabolism

Substances

  • Platelet-Derived Growth Factor
  • Transforming Growth Factor beta1
  • platelet-derived growth factor AB
  • Thrombin
  • Calcium Chloride