Changes in plasma endocannabinoid levels in viscerally obese men following a 1 year lifestyle modification programme and waist circumference reduction: associations with changes in metabolic risk factors

Diabetologia. 2009 Feb;52(2):213-7. doi: 10.1007/s00125-008-1178-6.


Aims/hypothesis: We previously reported that the plasma levels of the endocannabinoid, 2-arachidonoylglycerol (2-AG), in a cohort of viscerally obese men are directly correlated with visceral adipose tissue (VAT) accumulation and metabolic risk factors including low HDL-cholesterol and high triacylglycerol. It is not known, however, if such correlations persist after vigorous lifestyle interventions that reduce metabolic risk factors. We analysed the changes in endocannabinoid levels in a subsample from the same cohort following a 1 year lifestyle modification programme, and correlated them with changes in VAT and metabolic risk factors.

Methods: Forty-nine viscerally obese men (average age 49 years, BMI 30.9 kg/m(2), waist 107.3 cm) underwent a 1 year lifestyle modification programme including healthy eating and physical activity. Plasma levels of 2-AG and the other most studied endocannabinoid, anandamide, were measured by liquid chromatography-mass spectrometry. Anthropometric and metabolic risk factors, including VAT, insulin resistance and glucose intolerance, HDL-cholesterol and triacylglycerol, were measured.

Results: Most risk factors were improved by the intervention, which led to a significant decrease in body weight (-6.4 kg, p < 0.0001), waist circumference (-8.0 cm, p < 0.0001) and VAT (-30%, p < 0.0001), and in plasma 2-AG (-62.3%, p < 0.0001) and anandamide (-7.1%, p = 0.005) levels. The decrease in levels of 2-AG but not those of anandamide correlated with decreases in VAT and triacylglycerol levels, and with the increase in HDL(3)-cholesterol levels. Multivariate analyses suggested that decreases in 2-AG and VAT were both independently associated with decreases in triacylglycerol.

Conclusions/interpretation: This study shows that a strong correlation exists between 2-AG levels and high plasma triacylglycerol and low HDL(3)-cholesterol in viscerally obese men.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adiponectin / blood
  • Adipose Tissue / anatomy & histology
  • Apolipoproteins / blood
  • Arachidonic Acids / blood*
  • Body Mass Index
  • Body Weight
  • C-Reactive Protein / metabolism
  • Endocannabinoids
  • Glycerides / blood*
  • Humans
  • Interleukin-6 / blood
  • Leptin / blood
  • Life Style*
  • Lipids / blood
  • Male
  • Obesity / blood*
  • Obesity / rehabilitation*
  • Risk Factors
  • Triglycerides / blood
  • Waist Circumference
  • Weight Loss


  • Adiponectin
  • Apolipoproteins
  • Arachidonic Acids
  • Endocannabinoids
  • Glycerides
  • Interleukin-6
  • Leptin
  • Lipids
  • Triglycerides
  • glyceryl 2-arachidonate
  • C-Reactive Protein