The effect of iNOS inhibitors and hyperbaric oxygen treatment in a rat model of experimental colitis

Dig Dis Sci. 2009 Jan;54(1):75-9. doi: 10.1007/s10620-008-0498-1. Epub 2008 Oct 30.


Aim: Our aim was to investigate the effectiveness of aminoguanidine (AMG), an inducible nitric oxide synthase inhibitor, and hyperbaric oxygen (HBO) treatment in an experimental colitis model.

Methods: We induced colitis in rats. In the control group, we applied 2 ml serum physiologic intraperitoneally for 7 days. In the HBO group, 100% oxygen at 2.4 atm pressure was applied for 7 days. In the AMG group, 100 mg/kg AMG was applied intraperitoneally for 7 days. In the HBO + AMG group, HBO and AMG were applied, respectively. At the end of 7 days, rats were sacrificed and the distal 10 cm part of colon was examined macro- and microscopically.

Results: Severity of colitis and NO activities were reduced by AMG, HBO, and HBO + AMG application. There was histologically significant improvement, especially in the HBO + AMG group.

Conclusions: Both HBO and AMG were significantly effective in preventing weight loss, reducing NO activities, and severity of colitis, when comparing HBO and AMG separately.

MeSH terms

  • Acetic Acid
  • Animals
  • Colitis / chemically induced
  • Colitis / metabolism
  • Colitis / therapy*
  • Combined Modality Therapy
  • Disease Models, Animal
  • Female
  • Guanidines / therapeutic use*
  • Hyperbaric Oxygenation / methods*
  • Nitric Oxide / metabolism
  • Nitric Oxide Synthase Type II / antagonists & inhibitors*
  • Rats
  • Rats, Sprague-Dawley
  • Severity of Illness Index
  • Treatment Outcome


  • Guanidines
  • Nitric Oxide
  • Nitric Oxide Synthase Type II
  • Acetic Acid
  • pimagedine