Cyclic peptides from Oldenlandia affinis DC. Molecular and biological properties

Chem Biodivers. 2008 Oct;5(10):2014-22. doi: 10.1002/cbdv.200890184.

Abstract

A new isolation procedure for Kalata polypeptides from the tropical plant Oldenlandia affinis DC is described. Fractions were screened by thin-layer chromatography, and Van Urk positive fractions were tested for oxytocic activity in estrogenized rat uteri. By using this procedure, we were able to isolate and characterize three macrocyclic polypeptides with uterine activity. Their amino acid sequence and biological effects have been analyzed, and their NMR spectra were compared with those of the earlier ones. All three peptides showed hemolytic activity on human blood, and were tested for antibiotic effect against E. coli, Staphylococcus aureus, and Hemophilus influenzae.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Anti-Bacterial Agents* / chemistry
  • Anti-Bacterial Agents* / isolation & purification
  • Anti-Bacterial Agents* / pharmacology
  • Erythrocytes / cytology
  • Erythrocytes / drug effects
  • Escherichia coli / drug effects
  • Escherichia coli / growth & development
  • Female
  • Haemophilus influenzae / drug effects
  • Haemophilus influenzae / growth & development
  • Hemolysis / drug effects
  • Hemolytic Agents* / chemistry
  • Hemolytic Agents* / isolation & purification
  • Hemolytic Agents* / pharmacology
  • Humans
  • In Vitro Techniques
  • Magnetic Resonance Spectroscopy
  • Molecular Sequence Data
  • Oldenlandia / chemistry*
  • Oxytocics* / chemistry
  • Oxytocics* / isolation & purification
  • Oxytocics* / pharmacology
  • Peptides, Cyclic* / chemistry
  • Peptides, Cyclic* / isolation & purification
  • Peptides, Cyclic* / pharmacology
  • Protein Conformation
  • Rats
  • Staphylococcus aureus / drug effects
  • Staphylococcus aureus / growth & development
  • Uterine Contraction / drug effects
  • Uterus / drug effects

Substances

  • Anti-Bacterial Agents
  • Hemolytic Agents
  • Oxytocics
  • Peptides, Cyclic