The 29 medical records about acupuncture and moxibustion in Zhenjiu Dacheng are involved in the internal, surgical, gynecological, pediatric and five sense organs diseases and symptoms. Analysis on characteristics of these medical records and absorbing experiences of predecessors can better guide clinical application. Analysis of results indicates that these medical records have the characteristics of combination of acupuncture, moxibustion and medicine, less and better point selection, and stressing reinforcing-reducing of manipulation, at the same time, it reflects YANG's train of thought in clinical diagnosis and treatment of acupuncture and moxibustion.