Intracerebral development of transplanted glioblastoma C6 cells in rats after preliminary exposure to neuropeptides and an MAPK inhibitor

Neurosci Behav Physiol. 2008 Nov;38(9):913-6. doi: 10.1007/s11055-008-9072-8. Epub 2008 Oct 31.


The invasive growth, proliferation, and transcriptional regulation of glioma C6 cells treated with endothelin-1 and PD98059, a specific inhibitor of ERK1/2 were studied. The proliferation of glioma C6 cells was assessed in different growth conditions by prior in vitro treatment with endothelin-1 followed by implantation into the brain. In vitro studies showed that PD98059 inhibited the proliferation of cultured glioma C6 cells and activated transcription factors E2F1 and Myc-Max. Endothelin-1 significantly increased the proliferation of glioma C6 cells. The model used in this study was experimental and may allow the specific features of the in vitro behavior of cultured invasive cells to be identified.

MeSH terms

  • Animals
  • Basic-Leucine Zipper Transcription Factors / metabolism
  • Brain Neoplasms / metabolism*
  • Brain Neoplasms / pathology*
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Cells, Cultured
  • E2F1 Transcription Factor / metabolism
  • Endothelin-1 / pharmacology*
  • Flavonoids / pharmacology*
  • Glioblastoma / metabolism*
  • Glioblastoma / pathology*
  • Mitogen-Activated Protein Kinase Kinases / antagonists & inhibitors
  • Neoplasm Invasiveness / prevention & control
  • Neoplasm Transplantation
  • Proto-Oncogene Proteins c-myc / metabolism
  • Rats
  • Rats, Wistar


  • Basic-Leucine Zipper Transcription Factors
  • E2F1 Transcription Factor
  • E2f1 protein, rat
  • Endothelin-1
  • Flavonoids
  • MYC protein, human
  • Myc associated factor X
  • Proto-Oncogene Proteins c-myc
  • Mitogen-Activated Protein Kinase Kinases
  • 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one