Curcumin supplementation lowers TNF-alpha, IL-6, IL-8, and MCP-1 secretion in high glucose-treated cultured monocytes and blood levels of TNF-alpha, IL-6, MCP-1, glucose, and glycosylated hemoglobin in diabetic rats

Antioxid Redox Signal. 2009 Feb;11(2):241-9. doi: 10.1089/ars.2008.2140.

Abstract

This study examined the hypothesis that curcumin supplementation decreases blood levels of IL-6, MCP-1, TNF-alpha, hyperglycemia, and oxidative stress by using a cell-culture model and a diabetic rat model. U937 monocytes were cultured with control (7 mM) and high glucose (35 mM) in the absence or presence of curcumin (0.01-1 microM) at 37 degrees C for 24 h. Diabetes was induced in Sprague-Dawley rats by injection of streptozotocin (STZ) (i.p., 65 mg/kg BW). Control buffer, olive oil, or curcumin (100 mg/kg BW) supplementation was administered by gavage daily for 7 weeks. Blood was collected by heart puncture with light anesthesia. Results show that the effect of high glucose on lipid peroxidation, IL-6, IL-8, MCP-1, and TNF-alpha secretion was inhibited by curcumin in cultured monocytes. In the rat model, diabetes caused a significant increase in blood levels of IL-6, MCP-1, TNF-alpha, glucose, HbA(1), and oxidative stress, which was significantly decreased in curcumin-supplemented rats. Thus, curcumin can decrease markers of vascular inflammation and oxidative stress levels in both a cell-culture model and in the blood of diabetic rats. This suggests that curcumin supplementation can reduce glycemia and the risk of vascular inflammation in diabetes.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Blood Glucose / drug effects
  • Chemokine CCL2 / blood
  • Chemokine CCL2 / metabolism*
  • Curcumin / pharmacology*
  • Diabetes Mellitus, Experimental / blood
  • Diabetes Mellitus, Type 1 / blood
  • Glucose / pharmacology
  • Glycated Hemoglobin A / metabolism
  • Humans
  • Interleukin-6 / blood
  • Interleukin-6 / metabolism*
  • Interleukin-8 / metabolism*
  • Male
  • Monocytes / drug effects*
  • Monocytes / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Tumor Necrosis Factor-alpha / blood
  • Tumor Necrosis Factor-alpha / metabolism*
  • U937 Cells

Substances

  • Blood Glucose
  • Chemokine CCL2
  • Glycated Hemoglobin A
  • Interleukin-6
  • Interleukin-8
  • Tumor Necrosis Factor-alpha
  • Curcumin
  • Glucose