Abstract
To test the ability of high-throughput DNA sequencing to detect bacterial pathogens, we used it on DNA from a patient's feces during and after diarrheal illness. Sequences showing best matches for Campylobacter jejuni were detected only in the illness sample. Various bacteria may be detectable with this metagenomic approach.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Bacteria / isolation & purification*
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Bacterial Infections / diagnosis*
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Bacterial Typing Techniques / instrumentation
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Bacterial Typing Techniques / methods
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Campylobacter Infections / complications
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Campylobacter Infections / diagnosis
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Campylobacter jejuni / genetics
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Campylobacter jejuni / isolation & purification
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DNA, Bacterial / analysis*
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DNA, Bacterial / genetics
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Diagnosis, Differential
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Diarrhea / diagnosis
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Diarrhea / microbiology
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Feces / microbiology*
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Humans
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Male
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Reagent Kits, Diagnostic
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Sensitivity and Specificity
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Sequence Analysis, DNA / instrumentation
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Sequence Analysis, DNA / methods*
Substances
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DNA, Bacterial
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Reagent Kits, Diagnostic