Inhibition of human sperm function and mouse fertilization in vitro by an antibody against cation channel of sperm 1: the contraceptive potential of its transmembrane domains and pore region

Fertil Steril. 2009 Sep;92(3):1141-1146. doi: 10.1016/j.fertnstert.2008.07.1751. Epub 2008 Oct 31.


Objective: To explore the contraceptive potential of the CatSper1 transmembrane domains and pore region in vitro.

Design: In vitro study with human sperm and mouse fertilization.

Setting: Andrology laboratory of an academic research center.

Patient(s) and animal(s): Normozoospermia and viripotent BALB/c mice.

Intervention(s): The specific binding of an anti-CatSper1 IgG antibody (H-300) to CatSper1 was confirmed by Western blot and immunofluorescence. Sperm from humans and mice were incubated with H-300.

Main outcome measure(s): The effects of H-300 on human sperm progressive motility, abnormal acrosome, hyperactivated motility, and mouse in vitro fertilization rates were analyzed.

Result(s): A significant decline in sperm progressive motility was observed after 1, 2, and 4 hours of incubation with H-300; the change was mainly ascribed to the decline of fast progressive motility. Significant inhibition of the hyperactivated motility was observed after 5 hours of incubation with H-300. The incubation of mouse sperm with H-300 before insemination reduced the in vitro fertilization rate to 28% of control levels (72% inhibition).

Conclusion(s): CatSper1 may be a potential target for immunocontraception, and the antibody may be a tool to study the function of ion channels in sperm in which relatively fewer methods can be applied.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acrosome / drug effects
  • Animals
  • Antibodies, Anti-Idiotypic / immunology
  • Antibodies, Anti-Idiotypic / pharmacology
  • Calcium Channels / immunology*
  • Contraceptive Agents, Male / immunology
  • Contraceptive Agents, Male / pharmacology*
  • Female
  • Fertilization in Vitro / methods*
  • Humans
  • Immunoglobulin G / immunology
  • Immunoglobulin G / pharmacology*
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Porins / immunology*
  • Protein Binding
  • Protein Structure, Tertiary
  • Sperm Motility / drug effects
  • Sperm-Ovum Interactions / drug effects
  • Spermatozoa / drug effects*
  • Spermatozoa / physiology


  • Antibodies, Anti-Idiotypic
  • CATSPER1 protein, human
  • Calcium Channels
  • Catsper1 protein, mouse
  • Contraceptive Agents, Male
  • Immunoglobulin G
  • Porins