Spirulina platensis feeding inhibited the anemia- and leucopenia-induced lead and cadmium in rats

J Hazard Mater. 2009 May 30;164(2-3):1304-9. doi: 10.1016/j.jhazmat.2008.09.041. Epub 2008 Sep 20.


In the present investigation, the effect of Spirulina platensis (Sp) was undertaken on rats fed with lead and cadmium including diet by using physiological, enzymehistochemical and stereological methods. For this aim, 50 rats were equally divided into five groups as control (C), lead (Pb), Spirulina+lead (Sp+Pb), cadmium (Cd), and Spirulina+cadmium (Sp+Cd). Red blood cell (RBC) and white blood cell (WBC) counts, packed cell volume (PCV), and haemoglobine (Hb) concentrations were determined by haemocytometric methods in blood samples collected on 30th day. Population of T lymphocyte was counted by the alpha-naphthyl acetate esterase (ANAE) staining method, and reticulocytes were counted by stereological method. The counts of RBC, WBC, and ANAE positive T lymphocyte, and the values of Hb, PCV, and MCHC were decreased in the Pb and Cd groups compared to control group. Also, the number of reticulocytes (polychromatofilic erythrocyte) increased in the Pb groups, whereas it decreased in the Cd group. On the other hand, these values were ceased by S. platensis in the treated groups. These results suggest that S. platensis supplementation may be useful in adjuvant treatment of leukemia and anemia caused by lead and cadmium toxication.

MeSH terms

  • Anemia / chemically induced
  • Anemia / prevention & control*
  • Animals
  • Biological Therapy / methods*
  • Cadmium / adverse effects*
  • Diet
  • Hematologic Tests
  • Lead / adverse effects*
  • Leukopenia / chemically induced
  • Leukopenia / prevention & control*
  • Rats
  • Spirulina*
  • Treatment Outcome


  • Cadmium
  • Lead