Accordion-like Honeycombs for Tissue Engineering of Cardiac Anisotropy

Nat Mater. 2008 Dec;7(12):1003-10. doi: 10.1038/nmat2316. Epub 2008 Nov 2.

Abstract

Tissue-engineered grafts may be useful in myocardial repair; however, previous scaffolds have been structurally incompatible with recapitulating cardiac anisotropy. Here, we use microfabrication techniques to create an accordion-like honeycomb microstructure in poly(glycerol sebacate), which yields porous, elastomeric three-dimensional (3D) scaffolds with controllable stiffness and anisotropy. Accordion-like honeycomb scaffolds with cultured neonatal rat heart cells demonstrated utility through: (1) closely matched mechanical properties compared to native adult rat right ventricular myocardium, with stiffnesses controlled by polymer curing time; (2) heart cell contractility inducible by electric field stimulation with directionally dependent electrical excitation thresholds (p<0.05); and (3) greater heart cell alignment (p<0.0001) than isotropic control scaffolds. Prototype bilaminar scaffolds with 3D interconnected pore networks yielded electrically excitable grafts with multi-layered neonatal rat heart cells. Accordion-like honeycombs can thus overcome principal structural-mechanical limitations of previous scaffolds, promoting the formation of grafts with aligned heart cells and mechanical properties more closely resembling native myocardium.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Anisotropy
  • Biomechanical Phenomena
  • Decanoates / chemistry*
  • Electric Conductivity
  • Glycerol / analogs & derivatives*
  • Glycerol / chemistry
  • Heart / physiology*
  • Lasers, Excimer
  • Myocardium / cytology*
  • Myocardium / ultrastructure
  • Polymers / chemistry*
  • Rats
  • Tissue Engineering*
  • Tissue Scaffolds*
  • Ventricular Function

Substances

  • Decanoates
  • Polymers
  • poly(glycerol-sebacate)
  • Glycerol