Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comment
. 2008 Nov;10(11):1235-7; author reply 1237-40.
doi: 10.1038/ncb1108-1235.

UCPs--unlikely calcium porters

Paul S Brookes, Nadeene Parker, Julie A Buckingham, Antonio Vidal-Puig, Andrew P Halestrap, Thomas E Gunter, David G Nicholls, Paolo Bernardi, John J Lemasters, Martin D Brand
Comment

UCPs--unlikely calcium porters

Paul S Brookes et al. Nat Cell Biol. 2008 Nov.
No abstract available

PubMed Disclaimer

Figures

Figure 1
Figure 1. Ca2+ uptake by liver and heart mitochondria; effect of UCP inhibitors
Mitochondria were isolated from rat heart or liver as described in the supplemental information, and extra-mitochondrial [Ca2+] was measured using the Ca2+ sensitive dye Arsenazo III. Mitochondria were energized with succinate (complex II), with rotenone present to inhibit complex I. a. Representative Ca2+ traces for liver mitochondria. Each arrow represents addition of a Ca2+ bolus (20 µM final) to the incubation, resulting in an increase in extra-mitochondrial [Ca2+] followed by a decrease as Ca2+ is sequestered into mitochondria. Where indicated, inhibitors were present as follows: 1mM GDP (UCP inhibitor), 50 µM genipin (Gen, UCP2 inhibitor), 1 µM crude RR (MCU inhibitor). b. Quantitation of liver mitochondrial Ca2+ uptake rate (i.e. initial downward slope following Ca2+ spike), from experiments as shown in a. The average of second and third Ca2+ spikes from each trace was used for quantitation. Data are means ± SEM, N=3 (each N was an independent mitochondrial preparation on a separate day). c. As in b, data from heart mitochondria.
Figure 2
Figure 2. Ca2+ uptake in SkM mitochondria from wild-type (WT) and Ucp3−/− mice, plus liver and kidney mitochondria from wild-type and Ucp2−/− mice
Mitochondria were isolated and Ca2+ uptake measured as described in the supplemental information. a. Representative Ca2+ uptake traces in mitochondria from WT (blue) and Ucp3−/− (red) mouse SkM. Ca2+ (10 µM), FCCP (1 µM), and EGTA (1 mM) were added as indicated by arrows. Each trace is an average of 3 repeats on 1 preparation (N=1). b. Ca2+ uptake rates calculated from initial downward slopes of Ca2+ traces in a. The average of first and second Ca2+ spikes from each trace was used for quantitation. Data are means ± SEM, N=3 (where each N was an independent mitochondrial preparation on a separate day). There was no significant difference between WT and Ucp3−/− (Student’s t test). c. Ca2+ uptake studies in mitochondria isolated from WT (blue) and Ucp2−/− (red) mouse liver. Each trace is an average of 3 repeats on 1 preparation (N=1). This experiment is essentially identical in design to that shown in Figure 3 of [2], but gives a different result. d. Ca2+ uptake studies in mitochondria isolated from WT (blue) and Ucp2−/− (red) mouse kidney. Each trace is an average of 3 repeats on 1 preparation (N=1). N.B. in all panels, open/filled symbols represent the absence/presence, respectively, of crude RR (10 µM).
See this image and copyright information in PMC

Comment on

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Kirichok Y, Krapivinsky G, Clapham DE. Nature. 2004;427:360–364. - PubMed
    1. Trenker M, Malli R, Fertschai I, Levak-Frank S, Graier WF. Nature Cell Biol. 2007;9:445–452. - PMC - PubMed
    1. Graier WF, Frieden M, Malli R. Pflugers Arch. 2007;455:375–396. - PMC - PubMed
    1. Gunter TE, Buntinas L, Sparagna G, Eliseev R, Gunter K. Cell Calcium. 2000;28:285–296. - PubMed
    1. Stuart JA, Harper JA, Brindle KM, Jekabsons MB, Brand MD. Biochem. J. 2001;356:779–789. - PMC - PubMed