Injection of dsRNA into female A. aegypti mosquitos

J Vis Exp. 2007;(5):215. doi: 10.3791/215. Epub 2007 Jul 4.

Abstract

Reverse genetic approaches have proven extremely useful for determining which genes underly resistance to vector pathogens in mosquitoes. This video protocol illustrates a method used by the James lab to inject dsRNA into female A. aegypti mosquitoes, which harbor the dengue virus. The technique for calibrating injection needles, manipulating the injection setup, and injecting dsRNA into the thorax is illustrated.

Publication types

  • Video-Audio Media

MeSH terms

  • Aedes* / virology
  • Animals
  • Calibration
  • Dengue Virus / isolation & purification
  • Female
  • Microinjections / methods*
  • Needles
  • RNA, Double-Stranded / administration & dosage*
  • Thorax

Substances

  • RNA, Double-Stranded