TP53 Gene Mutations in Canine Osteosarcoma

Vet Surg. 2008 Jul;37(5):454-60. doi: 10.1111/j.1532-950X.2008.00407.x.

Abstract

Objective: To investigate mutations of the TP53 gene in canine osteosarcoma (OS).

Study design: Clinical historic cohort study.

Animals: Client-owned dogs.

Methods: OS (n=59) were screened for mutations of the complete TP53 gene using polymerase chain reaction and the mutation was analyzed by single-strand conformational polymorphism. Clinical outcome of dogs with TP53-mutated OS were compared with dogs with OS without a mutation after complete surgical excision of the primary tumor.

Results: TP53 gene mutations were observed in 24 of 59 (40.7%) OS; 3 mutated OS had 2 mutations. The alterations consisted mainly of point mutations (74%). Dogs with mutated OS had a significantly shorter survival time (ST) after surgery than dogs with normal tumor TP53 gene expression (P=.03). Other significant prognosticators for ST and disease-free interval included elevated serum alkaline phosphatase (P<.01) and tumor grade (P=.01).

Conclusion: TP53 genetic mutations are common in canine OS and may have a prognostic value.

Clinical relevance: Mutations of the TP53 gene may influence survival and should be considered when evaluating canine OS.

MeSH terms

  • Alkaline Phosphatase / metabolism
  • Animals
  • Biomarkers, Tumor
  • Bone Neoplasms / genetics
  • Bone Neoplasms / mortality
  • Bone Neoplasms / surgery
  • Bone Neoplasms / veterinary*
  • Disease-Free Survival
  • Dog Diseases / genetics*
  • Dog Diseases / mortality*
  • Dog Diseases / surgery
  • Dogs
  • Female
  • Genes, p53* / genetics
  • Kaplan-Meier Estimate
  • Male
  • Mutation
  • Neoplasm Staging / veterinary
  • Osteosarcoma / genetics
  • Osteosarcoma / mortality
  • Osteosarcoma / surgery
  • Osteosarcoma / veterinary*
  • Point Mutation
  • Polymerase Chain Reaction / methods
  • Polymerase Chain Reaction / veterinary*
  • Polymorphism, Single-Stranded Conformational
  • Treatment Outcome

Substances

  • Biomarkers, Tumor
  • Alkaline Phosphatase