Pluronic F-127 as a cell carrier for bone tissue engineering

J Biomater Appl. 2009 Sep;24(3):275-87. doi: 10.1177/0885328208096534. Epub 2008 Nov 5.


The objective of this study is to report the effect of Pluronic F-127 on osteoblast viability and phenotype maintenance in vitro. MG-63 cells are suspended in Pluronic F-127, and MTT assay, alkaline phosphatase activity, prostaglandin E(2) production, collagen-I, and cyclo-oxygenase-2 expression are assessed up to 6 days. Pluronic F-127 leads to a significant decrease in osteoblast viability throughout the 6-day experiment, without altering osteoblast phenotype. The addition of platelet-rich plasma to the polymer/cell construct leads to increased cell survival. When supplemented with bioactive factors, Pluronic F-127 could potentially be used as a cell carrier in bone tissue engineering.

MeSH terms

  • Animals
  • Bone Substitutes / chemistry*
  • Cell Line
  • Cell Proliferation
  • Cell Survival
  • Materials Testing
  • Mice
  • Osteoblasts / cytology
  • Osteoblasts / physiology*
  • Osteoblasts / transplantation*
  • Poloxamer / chemistry*
  • Tissue Engineering / methods*


  • Bone Substitutes
  • Poloxamer