Biliary 1-hydroxypyrene as a biomarker of exposure to polycyclic aromatic hydrocarbons in fish

Neuro Endocrinol Lett. 2008 Oct;29(5):663-8.

Abstract

Objectives: The aim of this study was the assessment of the Svitava and Svratka rivers contamination by polycyclic aromatic hydrocarbons (PAH) using 1-hydroxypyrene (1-OHP) concentrations in fish bile as a biomarker.

Design: Levels of 1-OHP were determined by reverse phase HPLC with fluorescence detection. For valid assessment of bile accumulation levels, the 1-OHP concentration was normalized to the biliary protein content. The content of 1-OHP was correlated with the PAH content of river bottom sediments.

Results: The highest level of 1-OHP in fish bile (136.1 ng.mg-1 protein) was found at the locality Rajhradice, which is situated downstream of Brno. Also the greatest level of sum of PAHs was found at this locality (17.1 microg.g-1 dry mass). Significant positive correlation (p< or =0.05) between the level of 1-OHP and sum of PAHs in sediment was found only in case of sediments collected in the same month as fish samples (in June).

Conclusion: Our results document that 1-OHP in fish bile is a suitable biochemical marker for the assessment of aquatic ecosystem contamination by PAHs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bile / metabolism*
  • Biomarkers
  • Chromatography, High Pressure Liquid
  • Cyprinidae / physiology
  • Czech Republic
  • Environmental Monitoring
  • Fishes / metabolism*
  • Fresh Water
  • Mutagens / metabolism*
  • Polycyclic Aromatic Hydrocarbons / analysis
  • Polycyclic Aromatic Hydrocarbons / toxicity*
  • Proteins / metabolism
  • Pyrenes / metabolism*
  • Spectrometry, Fluorescence
  • Water Pollutants, Chemical / analysis
  • Water Pollutants, Chemical / toxicity*

Substances

  • Biomarkers
  • Mutagens
  • Polycyclic Aromatic Hydrocarbons
  • Proteins
  • Pyrenes
  • Water Pollutants, Chemical
  • 1-hydroxypyrene