The MIRA method for DNA methylation analysis

Methods Mol Biol. 2009;507:65-75. doi: 10.1007/978-1-59745-522-0_6.

Abstract

DNA methylation patterns are often altered in human cancer and aberrant methylation is considered a hallmark of malignant transformation. Several methods have been developed for the characterization of gene-specific and genome-wide DNA methylation patterns. In this chapter, we describe the methylated-CpG island recovery assay (MIRA), which is based on the high affinity of the MBD2b/MBD3L1 complex for double-stranded CpG-methylated DNA. MIRA has been used in combination with microarray platforms to map DNA methylation patterns across the human genome.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • CpG Islands*
  • DNA / chemistry
  • DNA / isolation & purification
  • DNA Methylation*
  • DNA-Binding Proteins
  • Humans
  • Oligonucleotide Array Sequence Analysis / methods*
  • Recombinant Fusion Proteins
  • Repressor Proteins

Substances

  • DNA-Binding Proteins
  • MBD2 protein
  • MBD3L1 protein, human
  • Recombinant Fusion Proteins
  • Repressor Proteins
  • DNA