Micro-dissection of rat brain for RNA or protein extraction from specific brain region

J Vis Exp. 2007;(7):269. doi: 10.3791/269. Epub 2007 Aug 30.

Abstract

Micro-dissection of rat brain into various regions is extremely important for the study of different neurodegenerative diseases. This video demonstrates micro-dissection of four major brain regions include olfactory bulb, frontal cortex, striatum and hippocampus in fresh rat brain tissue. Useful tips for quick removal of respective regions to avoid RNA and protein degradation of the tissue are given.

Publication types

  • Video-Audio Media

MeSH terms

  • Animals
  • Brain / metabolism*
  • Brain / surgery
  • Corpus Striatum / metabolism
  • Frontal Lobe / metabolism
  • Hippocampus / metabolism
  • Microdissection / methods*
  • Nerve Tissue Proteins / isolation & purification*
  • Olfactory Bulb / metabolism
  • RNA / isolation & purification*
  • Rats
  • Specimen Handling / methods*

Substances

  • Nerve Tissue Proteins
  • RNA