Molecular characterization, chromosomal localization and association analysis with back-fat thickness of porcine LPIN2 and LPIN3

Mol Biol Rep. 2009 Sep;36(7):1819-24. doi: 10.1007/s11033-008-9385-2. Epub 2008 Nov 7.


The products of mammalian LPIN2 and LPIN3 are phosphatidate phosphatase type 1 enzymes, which play an important role in the de novo biosynthesis of triacylglycerol, phosphatidylcholine and phosphatidylethanolamine. In this study, we obtained a 2,985-bp cDNA sequence of porcine LPIN2, which contains a 2,676-bp open reading frame flanked by an 11-bp 5'UTR and a 298-bp 3'UTR, and a 2,843-bp cDNA sequence of porcine LPIN3, which contains a 111-bp 5'UTR, a 2,580-bp open reading frame and a 152-bp 3'UTR. RT-PCR analysis showed that both LPIN2 and LPIN3 mRNA were ubiquitously expressed with a very high level in liver. By using the somatic cell hybrid panel (SCHP) and the radiation hybrid (IMpRH) panel, porcine LPIN2 and LPIN3 were assigned to 6q24-(1/2)q31 and 17(1/2)q21-q23, respectively. One T2193C single nucleotide polymorphism in LPIN2 was identified and was detected by Hin6I PCR-RFLP. Association analysis showed that different genotypes of LPIN2 were associated with back-fat thickness between the 6th and 7th ribs (P < 0.01).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adiposity / genetics*
  • Animals
  • Chromosomes, Mammalian / metabolism*
  • DNA, Complementary / genetics
  • Gene Expression Profiling
  • Gene Expression Regulation
  • Genotype
  • Nuclear Proteins / genetics*
  • Nuclear Proteins / metabolism
  • Phylogeny
  • Polymorphism, Single Nucleotide / genetics
  • Sequence Homology, Nucleic Acid
  • Sus scrofa / genetics*


  • DNA, Complementary
  • Nuclear Proteins