Crystal structures of protein phosphatase-1 bound to nodularin-R and tautomycin: a novel scaffold for structure-based drug design of serine/threonine phosphatase inhibitors

J Mol Biol. 2009 Jan 9;385(1):11-21. doi: 10.1016/j.jmb.2008.10.053. Epub 2008 Nov 1.

Abstract

Protein phosphatase 1 occurs in all tissues and regulates many pathways, ranging from cell-cycle progression to carbohydrate metabolism. Many naturally occurring, molecular toxins modulate PP1 activity, though the exact mechanism of this differential regulation is not understood. A detailed elucidation of these interactions is crucial for understanding the cellular basis of phosphatase function and signaling pathways but, more importantly, they can serve as the basis for highly specific therapeutics, e.g. against cancer. We report the crystal structures of PP1 in complex with nodularin-R at 1.63 A and tautomycin at 1.70 A resolution. The PP1:nodularin-R complex was used to demonstrate the utility of our improved PP1 production technique, which produces highly active, soluble PP1. Tautomycin is one of the few toxins that reportedly preferentially binds PP1>PP2A. Therefore, the PP1:tautomycin structure is the first complex structure with a toxin with preferred PP1 specificity. Furthermore, since tautomycin is a linear non-peptide-based toxin, our reported structure will aid the design of lead compounds for novel PP1-specific pharmaceuticals.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Binding Sites
  • Cantharidin / chemistry
  • Crystallography, X-Ray
  • Drug Design*
  • Enzyme Inhibitors / chemistry*
  • Enzyme Inhibitors / pharmacology
  • Humans
  • Hydrogen Bonding
  • Models, Molecular
  • Peptides, Cyclic / chemistry*
  • Protein Phosphatase 1 / antagonists & inhibitors
  • Protein Phosphatase 1 / chemistry*
  • Protein Structure, Secondary
  • Pyrans / chemistry*
  • Spiro Compounds / chemistry*
  • Structure-Activity Relationship

Substances

  • Enzyme Inhibitors
  • Peptides, Cyclic
  • Pyrans
  • Spiro Compounds
  • nodularin
  • tautomycin
  • Protein Phosphatase 1
  • Cantharidin

Associated data

  • PDB/3E7A
  • PDB/3E7B