Crystallization and preliminary X-ray analysis of the O-carbamoyltransferase NovN from the novobiocin-biosynthetic cluster of Streptomyces spheroides

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Nov 1;64(Pt 11):1000-2. doi: 10.1107/S1744309108030145. Epub 2008 Oct 25.


Crystals of recombinant NovN, an O-carbamoyltransferase from Streptomyces spheroides, were grown by vapour diffusion. The protein crystallized in two different crystal forms. Crystal form I belonged to space group C2 and native data were collected to 2.9 A resolution in-house. Crystal form II had I-centred orthorhombic symmetry and native data were recorded to a resolution of 2.3 A at a synchrotron. NovN catalyses the final step in the biosynthesis of the aminocoumarin antibiotic novobiocin that targets the essential bacterial enzyme DNA gyrase.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / chemistry*
  • Bacterial Proteins / genetics
  • Carboxyl and Carbamoyl Transferases / chemistry*
  • Carboxyl and Carbamoyl Transferases / genetics
  • Crystallization
  • Crystallography, X-Ray
  • Enzyme Inhibitors / metabolism
  • Molecular Sequence Data
  • Multigene Family
  • Novobiocin / biosynthesis*
  • Streptomyces / enzymology*


  • Bacterial Proteins
  • Enzyme Inhibitors
  • Novobiocin
  • Carboxyl and Carbamoyl Transferases