Isolation of herbicide-resistant 4-hydroxyphenylpyruvate dioxygenase from cultured Coptis japonica cells

Biosci Biotechnol Biochem. 2008 Nov;72(11):3059-62. doi: 10.1271/bbb.80466. Epub 2008 Nov 7.

Abstract

4-Hydroxyphenylpyruvate dioxygenase (HPPD) catalyzes the formation of homogentisate from 4-hydroxyphenylpyruvate and O(2). In plants, HPPD has been identified as a molecular target for herbicides. We report the isolation and characterization of a cDNA encoding a HPPD from cultured Coptis japonica cells. Recombinant CjHPPD showed significantly higher half-maximum inhibitory concentration (IC(50)) values for the HPPD-inhibiting herbicide destosyl pyrazolate than other plant HPPDs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 4-Hydroxyphenylpyruvate Dioxygenase / antagonists & inhibitors
  • 4-Hydroxyphenylpyruvate Dioxygenase / genetics
  • 4-Hydroxyphenylpyruvate Dioxygenase / isolation & purification*
  • 4-Hydroxyphenylpyruvate Dioxygenase / metabolism*
  • Amino Acid Sequence
  • Berberine / metabolism
  • Cells, Cultured
  • Coptis / cytology*
  • Coptis / drug effects
  • Coptis / enzymology*
  • Coptis / metabolism
  • Gene Library
  • Herbicide Resistance
  • Herbicides / pharmacology*
  • Humans
  • Molecular Sequence Data
  • Pyrazoles / pharmacology

Substances

  • Herbicides
  • Pyrazoles
  • destosyl pyrazolate
  • Berberine
  • 4-Hydroxyphenylpyruvate Dioxygenase