Greatly reduced amino acid alphabets in directed evolution: making the right choice for saturation mutagenesis at homologous enzyme positions

Chem Commun (Camb). 2008 Nov 21:(43):5499-501. doi: 10.1039/b813388c. Epub 2008 Oct 2.

Abstract

Enantioselective mutants of the thermally robust phenyl acetone monooxygenase (PAMO) as catalysts in Baeyer-Villiger reactions have been evolved by utilizing saturation mutagenesis in which drastically reduced amino acid alphabets are employed at homologous enzyme positions.

MeSH terms

  • Amino Acid Sequence
  • Amino Acids / chemistry*
  • Biocatalysis
  • Cytochrome P-450 Enzyme System / chemistry*
  • Cytochrome P-450 Enzyme System / genetics
  • Cytochrome P-450 Enzyme System / metabolism*
  • Directed Molecular Evolution*
  • Ketones / chemistry
  • Kinetics
  • Molecular Sequence Data
  • Molecular Structure
  • Mutagenesis*
  • Oxepins / chemical synthesis
  • Oxepins / chemistry
  • Oxidation-Reduction
  • Sequence Alignment
  • Stereoisomerism

Substances

  • Amino Acids
  • Ketones
  • Oxepins
  • Cytochrome P-450 Enzyme System
  • acetone monooxygenase