Atlas of Gene Expression in the Developing Kidney at Microanatomic Resolution

Dev Cell. 2008 Nov;15(5):781-91. doi: 10.1016/j.devcel.2008.09.007.

Abstract

Kidney development is based on differential cell-type-specific expression of a vast number of genes. While multiple critical genes and pathways have been elucidated, a genome-wide analysis of gene expression within individual cellular and anatomic structures is lacking. Accomplishing this could provide significant new insights into fundamental developmental mechanisms such as mesenchymal-epithelial transition, inductive signaling, branching morphogenesis, and segmentation. We describe here a comprehensive gene expression atlas of the developing mouse kidney based on the isolation of each major compartment by either laser capture microdissection or fluorescence-activated cell sorting, followed by microarray profiling. The resulting data agree with known expression patterns and additional in situ hybridizations. This kidney atlas allows a comprehensive analysis of the progression of gene expression states during nephrogenesis, as well as discovery of potential growth factor-receptor interactions. In addition, the results provide deeper insight into the genetic regulatory mechanisms of kidney development.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Embryo, Mammalian / metabolism
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental*
  • Kidney / embryology*
  • Kidney / metabolism*
  • Mice
  • Mice, Inbred Strains
  • Nephrons / embryology
  • Nephrons / metabolism
  • Oligonucleotide Array Sequence Analysis