A new class of reversible cell cycle inhibitors

Cytometry. 1991;12(1):26-32. doi: 10.1002/cyto.990120105.


The effects of three compounds on the cell cycle of HL-60 promyeloid leukemia cells has been examined. Ciclopirox olamine, an antifungal agent, and the compound Hoechst 768159 reversibly block the cell cycle at a point occurring roughly 1 h before the arrest mediated by aphidicolin, an inhibitor of DNA polymerase alpha activity, which acts in early S phase. Similar results are also obtained with the compound mimosine, a plant amino acid. Based on these data, it is concluded that all three agents inhibit cell cycle traverse at or very near the G1/S phase boundary and identify a previously undefined reversible cell cycle arrest point.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antifungal Agents / pharmacology*
  • Aphidicolin
  • Cell Cycle / drug effects
  • Cell Line
  • Ciclopirox
  • DNA Polymerase II / antagonists & inhibitors*
  • Diterpenes / pharmacology*
  • G1 Phase / drug effects
  • Humans
  • Leukemia, Promyelocytic, Acute / pathology*
  • Mimosine / pharmacology*
  • Pyridones / pharmacology*
  • S Phase / drug effects
  • Thiazoles / pharmacology*
  • Tumor Cells, Cultured / drug effects
  • Tumor Cells, Cultured / pathology


  • Antifungal Agents
  • Diterpenes
  • Pyridones
  • Thiazoles
  • Ciclopirox
  • Aphidicolin
  • Mimosine
  • HOE 768159
  • DNA Polymerase II