Protective effect of vitamin C on oxidative stress: a randomized controlled trial

Int J Vitam Nutr Res. 2008 May;78(3):121-8. doi: 10.1024/0300-9831.78.3.121.


Background: Although a number of reports regarding the role of reactive oxygen species (ROS) as the first step in cancer induction exist, few studies have investigated how vitamin C influences ROS in human plasma.

Aim of the study: Using the ROS assay system, a method recently established by one of the authors, we aimed to evaluate the effect of vitamin C supplementation on serum ROS among subjects diagnosed with chronic gastritis.

Methods: A total of 244 Japanese subjects with atrophic gastritis were randomized to take 5-year supplementation of either 50 mg or 500 mg of vitamin C.

Results: The adjusted difference in the changes of total ROS between baseline and after 5-year supplementation was statistically significant between the intervention groups: 2.70 decrease (corresponds to 1.26% decrease) in the high-dose group and 4.16 increase (corresponds to 3.79% increase) in the low-dose group, p for difference = 0.01.

Conclusion: Vitamin C was suggested to reduce oxidative stress among subjects with atrophic gastritis.

Publication types

  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Analysis of Variance
  • Antioxidants / metabolism
  • Antioxidants / therapeutic use*
  • Ascorbic Acid / blood
  • Ascorbic Acid / therapeutic use*
  • Chronic Disease
  • Dietary Supplements
  • Dose-Response Relationship, Drug
  • Female
  • Gastritis, Atrophic / blood*
  • Gastritis, Atrophic / microbiology
  • Helicobacter Infections / blood
  • Helicobacter Infections / microbiology
  • Helicobacter pylori
  • Humans
  • Japan
  • Male
  • Middle Aged
  • Oxidative Stress / drug effects*
  • Reactive Oxygen Species / blood


  • Antioxidants
  • Reactive Oxygen Species
  • Ascorbic Acid